BIOMG 4400 - Laboratory in Biochemistry and Molecular Biology

Fall, spring, summer (Six-week session). 4 credits.

Prerequisites: BIOMG 3300, or BIOMG 3330, or BIOMG 3350, or BIOMG 3310/BIOMG 3320 (at least one of BIOMG 3310/BIOMG 3320 completed but one may be taken concurrently). Priority given to undergraduate biology majors in Biochemistry or Molecular and Cell Biology programs of study and to graduate students with a minor in field of biochemistry. Limited enrollment.

H. Nivison.

Experiments related to molecular biology (includes PCR, DNA cloning, hybridization analysis, restriction mapping, and DNA sequence analysis), protein purification and analysis (salt fractionation, ion exchange chromatography, affinity chromatography, SDS-PAGE, and immunoblotting), and determination of enzyme kinetic parameters.