NS 4900 - [Manipulating the Mouse Genome]

Fall (weeks 1-7). Next offered 2018-2019 (offered even alternate years). 1 credit. Student option grading.

Prerequisite: BIOMG 2800, BIOMG 3300, BIOMG 3320 or BIOMG 3330, or NS 3200. Meets during first half of semester and provides background information for VTBMS 7010/TOX 7010 Mouse Pathology and Transgenesis, which meets during second half. Students interested in both courses must register for them separately.

P. Soloway.

Functional genomics has relied upon tools for manipulating the mouse genome. This course explores those tools, including transgenesis, gene targeting using ES cells and CRISPR/cas, gene trapping, chemical mutagenesis, use of induced pluripotent stem cells, and cloning by nuclear transplant. Also discussed are use of recombinant inbred mice and genome-wide association studies for complex trait analysis.

Outcome 1: Upon completing this course, students will be able to design strategies using these methods to address practical problems in experimental mammalian genetics.