PLPPM 4250 - [DNA Targeting: Applications, Reagents, and Impact]

(OPHLS-AG)

Spring. Next Offered: 2021-2022. 3 credits. Student option grading.

Recommended Prerequisite: BIOMG 2800 and BIOMG 2801 or equivalent; BIOMG 3300, BIOMG 3310 with BIOMG 3320, BIOMG 3330, or BIOMG 3350; BIOMG 4000.

A. Bogdanove.

Recent advances in our capacity to direct molecules to specific DNA sequences in vitro and in living cells give us an unprecedented ability to probe and modify the genetic material. Through primary literature, this course considers applications of DNA targeting, ranging from systems biology to genome editing; reagents, including zinc finger proteins, TAL effectors, the CRISPR/Cas9 complex and others; and impact, reaching basic science, agriculture, and medicine. Regulatory, ethical, intellectual property, and public perception considerations are also discussed.

Outcome 1: Possess detailed knowledge and in depth understanding of major topics in the field of DNA targeting

Outcome 2: Critically evaluate research articles in the field of DNA targeting.

Outcome 3: Find, access, and manage relevant publications.