BIOMG 4400 - Laboratory in Biochemistry and Molecular Biology

(PBS)

Fall, spring. 4 credits. Letter grades only.

Prerequisite: BIOMG 3300, or BIOMG 3330, or BIOMG 3350, or BIOMG 3310/BIOMG 3320 (at least one of BIOMG 3310/BIOMG 3320 completed but one may be taken concurrently). Priority given to undergraduate biology majors in Biochemistry or Molecular and Cell Biology programs of study and to graduate students with a minor in field of biochemistry. Limited enrollment.

H. Nivison.

Experiments related to molecular biology (includes PCR, DNA cloning, hybridization analysis, restriction mapping, and DNA sequence analysis), protein purification and analysis (salt fractionation, ion exchange chromatography, affinity chromatography, SDS-PAGE, and immunoblotting), and determination of enzyme kinetic parameters.

Outcome 1: After completing BIOMG 4400, students should be able to: Plan, carry out, and interpret the results of laboratory experiments.

Outcome 2: Use modern laboratory techniques to purify and study proteins and to purify and work with nucleic acids.

Outcome 3: Explain the theoretical basis of the laboratory techniques.

Outcome 4: Use Internet databases to assist in data analysis.

Outcome 5: Create and use a laboratory notebook.

Outcome 6: Present results of laboratory work, both orally and in writing.