CHEM 6860 - Physical Chemistry of Proteins

Fall. 4 credits. Letter grades only.

Prerequisite: CHEM 3880 or CHEM 3900, or equivalents. Enrollment preference given to: graduate students.

P. Chen.

Physical studies of proteins, with emphasis on using single molecule methodologies and on studies of metalloproteins. Topics include: Physical/chemical concepts that include chemical structure and conformation of proteins, protein folding energy landscape, electron transfer theory, enzyme catalysis, chemical kinetics, and single-molecule kinetics. Experimental methodologies that include absorption and emission spectroscopy, fluorescence energy resonance transfer, confocal microscopy, total internal reflection fluorescence, single molecule spectroscopy, time correlated single photon counting, fluorescence correlation spectroscopy, atomic force microscopy, optical tweezers, magnetic tweezers, super-resolution imaging with optical microscopy. Protein structure and function that includes metalloprotein structure/function (bioinorganic chemistry), GFP and variants, protein labeling, motor proteins, protein-protein interactions, protein-DNA interactions, and live-cell imaging.