BIONB 4300 - Experimental Molecular Neurobiology

(PBS-AS)

Spring. 4 credits. Letter grades only.

Prerequisite: BIOMG 3300 or BIOMG 3310. Recommended prerequisite: BIOMG 2801. Enrollment limited to: 16 students.

D. L. Deitcher.

Experiments include PCR, immunoprecipitations, western blots, RNAi, antibody staining, optogenetics, and behavioral assays. Experiments emphasize how molecular techniques can be applied to studying neurobiological problems and will culminate in designing your own experiments.

Outcome 1: Demonstrate competence in basic molecular biology and protein biochemistry skills in the laboratory and the underlying principles of these techniques.

Outcome 2: Demonstrate mastery of basic bioinformatics tools such as BLASTN, TBLASTN, and BLASTP and using model organism databases such as FlyBase.

Outcome 3: Develop a clear understanding of how different laboratory techniques provide complementary information about gene expression.

Outcome 4: Demonstrate deep understanding of scientific procedures and results through written laboratory reports.