AEP 4700 - Biophysical Methods

(crosslisted) BIONB 4700, VETMM 4700

Fall. 3 credits. Student option grading.

Prerequisite: solid knowledge of basic physics and mathematics through sophomore level. Recommended prerequisite: some knowledge of cellular biology. Co-meets with AEP 5700/BME 5700.

G. Lambert.

Overview of the diversity of modern biophysical experimental techniques used in the study of biophysical systems at the molecular, cellular, and population level. Emphasis is placed on groundbreaking methods behind recent Nobel Prizes and other techniques likely to be encountered in cutting-edge research and industry. Topics include: 1) super-resolution, multi-photon, and single molecule microscopy, 2) crystallography and structural biology methods used to characterize DNA, RNA, proteins, cells, tissues, 3) microfluidics, “lab-on-a-chip”, and single cell culture techniques, 4) molecular dynamics simulations, stochastic modeling, and physical models of a cell, and 5) next-generation sequencing, protein engineering, synthetic biology, genome editing, and other experimental techniques at the intersection of applied physics and biological engineering.